The Ion Chromatography instrument includes a Metrohm 858 Autosampler with 127 positions for sample introduction. It also offers options for inline auto dilution and ultrafiltration of samples prior to analysis. The anions are quantified using a Metrohm IC 930 compact IC Flex Oven/SeS/PP/Deg, which is equipped with a column oven, MSC and MSM module for sequential suppression, a peristaltic pump for suppressor regeneration and a built-in degasser. Detection and quantification of anions can be achieved using either a conductivity detector and/or an UV/VIS detector. The cation is analysed using a Metrohm IC 930 compact IC Flex Oven/Deg equipped with a column oven and a built-in degasser. The cations are quantified using a Conductivity detector.
When a sample is injected in the system, the ions in the sample first separated on the columns by adhering to the stationary phase and the different ions elute at various times. The ions are measured using a conductivity detector. A calibration is prepared by measuring the conductivity response of known concentrations of ion standards. This technique can be used to quantify anions (Cl, F, Br, NO2, NO3, PO4, SO4) and Cations (Ca, Mg, Li, NH4, K, Na) in aqueous samples such as ground, surface, municipal, rain and seawater, soil extracts and effluent by implementing the calibration curve.
Stellenbosch University
Medical Campus Tygerberg,
Cape Town 7505 South Africa
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