Imaging Flow Cytometer

Equipment Details

Lab:

Flow Cytometry

Description:

An Imaging Flow Cytometer is an instrument that operates like a conventional flow cytometer; however, it has the added advantage of providing images of every event acquired. In this state-of-the-art technology, the speed and quantitative properties of flow cytometry is combined with the detailed imagery properties of microscopy, and it is all delivered via one unique instrument.

Model:

Cytek AMNIS® ImageStreamX Mk II Flow Cytometer

Location:

Biomedical Research Institute (BMRI), Tygerberg Medical campus

Functions:

Cytek AMNIS ® ImageStreamX Mk II Performance characteristics:

2 camera system, delivering images of brightfield, side scatter (SSC) and 9- 10 fluorescent channels.

Cytek AMNIS laser and bandpass configuration

Objectives: 20x objectives to view larger structures or cells, 40x and 60x objectives for the analyses of cells (as small as yeast or bacteria) or for defining intracellular detail of larger cells
Extended depth of field (EDF) delivering a single in focus image of a structure or cell
High throughput with an imaging rate per magnification as follows:

Magnification	Max acquisition rate
20x	5 000 cells/sec
40x	2 000 cells/sec
60x	1 200 cells/sec

Automated acquisition from 96 well round bottom plates
Manual acquisition from 1.5 ml microcentrifuge tubes or 96 well round bottom plates
Sample in suspension is required, able to analyse sample volumes between 20-200ul
High resolution images and statistics are retrieved in real-time
Automated instrument operations which includes start up, calibration and shut down
Enumeration of events (events/μl), which eliminates the need of using counting beads

Purpose:

Nuclear Translocation of NFkB, Intracellular molecular trafficking, Immune Synapse, Shape change, Phagocytosis, Autophagy, etc.

Equipment Details